Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Baba Gang Nath Marg, Munirka, New Delhi 110067, India; Department of Zoology, University of Delhi, New Delhi 110007, India.
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Baba Gang Nath Marg, Munirka, New Delhi 110067, India.
Mol Cell Endocrinol. 2014 Mar 5;383(1-2):10-20. doi: 10.1016/j.mce.2013.11.021. Epub 2013 Dec 4.
Quercetin, as a flavonoid, has been recognized to possess dual properties of an oxidant and antioxidant as well. The role of quercetin (QC), as an antioxidant in countering estradiol-3-benzoate (EB) induced adverse effects and germ cell apoptosis in adult rat testis was presently investigated. Adult rats received EB (0.075 mg/rat/5th day) alone or EB+QC (15 mg/kg bw/alternate day) simultaneously for 30 days. Revival of spermatogenesis following QC intervention was associated with a significant restoration in serum and intra-testicular levels of testosterone. Decline in lipid peroxidation and simultaneous improvement in the activities of superoxide dismutase, catalase and glutathione s-transferase were very much evident. Identically, total antioxidant capacity and glutathione demonstrated a marked improvement. QC augmented germ cell survival leading to a decrease in cell apoptosis. Expression of downstream apoptotic markers, caspase-3 and poly-ADP-ribose polymerase (PARP) presented a significant reduction. Down regulation with respect to upstream markers, caspase-8 and -9, Fas, FasL, Bax, and p53 was similarly observed. Taken together, the above findings indicate that with the dose presently used quercetin with its antioxidant and antiestrogenic properties restored testicular function leading to revival of spermatogenesis. It also augmented germ cell survival primarily mediated through downregulation in the expressions of upstream, downstream and other markers in the pathways of metazoan apoptosis.
槲皮素作为一种类黄酮，已被认为具有氧化剂和抗氧化剂的双重特性。本研究旨在探讨槲皮素（QC）作为抗氧化剂，对抗雌二醇-3-苯甲酸酯（EB）诱导的成年大鼠睾丸生殖细胞凋亡的作用。成年大鼠单独接受 EB（0.075mg/rat/5 天）或同时接受 EB+QC（15mg/kg bw/隔日）治疗 30 天。QC 干预后精子发生的恢复与血清和睾丸内睾酮水平的显著恢复有关。脂质过氧化的下降和超氧化物歧化酶、过氧化氢酶和谷胱甘肽 S-转移酶活性的同时改善非常明显。同样，总抗氧化能力和谷胱甘肽也有明显改善。QC 增加了生殖细胞的存活，导致细胞凋亡减少。下游凋亡标志物 caspase-3 和多聚 ADP-核糖聚合酶（PARP）的表达显著降低。上游标志物 caspase-8 和 -9、Fas、FasL、Bax 和 p53 的下调也观察到了同样的情况。综上所述，这些发现表明，目前使用的槲皮素剂量具有抗氧化和抗雌激素特性，可恢复睾丸功能，促进精子发生。它还通过下调凋亡途径中上游、下游和其他标志物的表达，增加生殖细胞的存活。
