Blecharz-Klin Kamilla, Joniec-Maciejak Ilona, Piechal Agnieszka, Pyrzanowska Justyna, Wawer Adriana, Widy-Tyszkiewicz Ewa
Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Krakowskie Przedmieście 26/28, 00-927 Warsaw, Poland.
Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Krakowskie Przedmieście 26/28, 00-927 Warsaw, Poland.
Environ Toxicol Pharmacol. 2014 Jan;37(1):95-102. doi: 10.1016/j.etap.2013.11.004. Epub 2013 Nov 13.
In our experiment we investigated the effect of subcutaneous administration of paracetamol on the levels of amino acids in the brain structures. Male Wistar rats received for eight weeks paracetamol at two doses: 10 mg/kg b.w. (group P10, n=9) and 50 mg/kg b.w. per day s.c. (group P50, n=9). The regional brain concentrations of amino acids were determined in the prefrontal cortex, hippocampus, hypothalamus and striatum of control (Con, n=9) and paracetamol-treated groups using HPLC. Evaluation of the biochemical results indicated considerable decrease of the content of amino acids in the striatum (glutamine, glutamic acid, taurine, alanine, aspartic acid) and hypothalamus (glycine) between groups treated with paracetamol compared to the control. In the prefrontal cortex paracetamol increased the level of γ-aminobutyric acid (GABA). The present study demonstrated significant effect of the long term paracetamol treatment on the level of amino acids in the striatum, prefrontal cortex and hypothalamus of rats.
在我们的实验中，我们研究了皮下注射扑热息痛对脑结构中氨基酸水平的影响。雄性Wistar大鼠连续八周皮下注射两种剂量的扑热息痛：10毫克/千克体重（P10组，n = 9）和50毫克/千克体重（P50组，n = 9）。使用高效液相色谱法（HPLC）测定对照组（Con，n = 9）和扑热息痛处理组大鼠前额叶皮质、海马体、下丘脑和纹状体中氨基酸的区域脑浓度。生化结果评估表明，与对照组相比，扑热息痛处理组的纹状体（谷氨酰胺、谷氨酸、牛磺酸、丙氨酸、天冬氨酸）和下丘脑（甘氨酸）中氨基酸含量显著降低。在前额叶皮质中，扑热息痛增加了γ-氨基丁酸（GABA）的水平。本研究表明，长期使用扑热息痛对大鼠纹状体、前额叶皮质和下丘脑的氨基酸水平有显著影响。
