Unité de Caractérisation des Toxines, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
UMR 7245 CNRS-MNHN Molécules de Communication et Adaptation des Micro-organismes, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.
Environ Pollut. 2014 Feb;185:333-9. doi: 10.1016/j.envpol.2013.10.016. Epub 2013 Dec 5.
So far only a few publications have explored the development of extraction methods of cyanotoxin extracted from complex matrices. With regard to cyanobacterial microcystins (MCs), the data on the contamination of the flesh of aquatic organisms is hard to compare and very limited due to the lack of validated methods. In recent years, evidence that both free and bound fractions of toxin are found in these tissues has highlighted the need to develop effective methods of quantification. Several techniques do exist, but only the Lemieux oxidation has so far been used to investigate complex tissue matrices. In this study, protocols based on the Lemieux approach were adapted for the quantitative chemical analysis of free MC-LR and MMPB derived from bound toxin in the tissues of juvenile trout gavaged with MC-LR. Afterwards, the NF V03 110 guideline was used to characterize the protocols elaborated and evaluate their effectiveness.
迄今为止，只有少数出版物探讨了从复杂基质中提取蓝藻毒素的提取方法的发展。关于蓝细菌微囊藻毒素（MCs），由于缺乏经过验证的方法，水生生物组织污染的数据很难进行比较且非常有限。近年来，有证据表明，这些组织中既存在游离毒素，也存在结合毒素，这凸显出需要开发有效的定量方法。目前已经存在几种技术，但到目前为止，只有莱米厄氧化法被用于研究复杂的组织基质。在这项研究中，根据莱米厄方法改编的方案被用于对用 MC-LR 灌胃的幼鳟鱼组织中结合毒素衍生的游离 MC-LR 和 MMPB 进行定量化学分析。然后，使用 NF V03 110 准则来描述所制定的方案并评估其有效性。
