Ramly Nur Zazarina, Rouzheinikov Sergey N, Sedelnikova Svetlana E, Baker Patrick J, Chow Yock Ping, Wan Kiew Lian, Nathan Sheila, Rice David W
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, England.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1380-3. doi: 10.1107/S1744309113029734. Epub 2013 Nov 29.
Coccidiosis in chickens is caused by the apicomplexan parasite Eimeria tenella and is thought to involve a role for a superfamily of more than 20 cysteine-rich surface antigen glycoproteins (SAGs) in host-parasite interactions. A representative member of the family, SAG19, has been overexpressed in Escherichia coli, purified and crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as the precipitant. Crystals of SAG19 diffracted to beyond 1.50 Å resolution and belonged to space group I4, with unit-cell parameters a = b = 108.2, c = 37.5 Å. Calculation of possible values of VM suggests that there is a single molecule in the asymmetric unit.
鸡球虫病由顶复门寄生虫柔嫩艾美耳球虫引起，据认为，超过20种富含半胱氨酸的表面抗原糖蛋白（SAGs）超家族在宿主与寄生虫的相互作用中发挥作用。该家族的一个代表性成员SAG19已在大肠杆菌中过表达，经纯化后，以硫酸铵作为沉淀剂，采用气相扩散悬滴法进行结晶。SAG19晶体的衍射分辨率超过1.50 Å，属于空间群I4，晶胞参数a = b = 108.2，c = 37.5 Å。VM可能值的计算表明，不对称单元中有一个单分子。
