金黄色葡萄球菌LytR蛋白接收结构域的表达、纯化、结晶及初步X射线分析
Expression, purification, crystallization and preliminary X-ray analysis of the receiver domain of Staphylococcus aureus LytR protein.
作者信息
Shala Agnesa, Patel Kevin H, Golemi-Kotra Dasantila, Audette Gerald F
机构信息
Department of Chemistry, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1418-21. doi: 10.1107/S1744309113030972. Epub 2013 Nov 29.
The response-regulatory protein LytR belongs to a family of transcription factors involved in the regulation of important virulence factors in pathogenic bacteria. The protein consists of a receiver domain and an effector domain, which play an important role in controlled cell death and lysis. The LytR receiver domain (LytR(N)) has been overexpressed, purified and crystallized using the sitting-drop and hanging-drop vapour-diffusion methods. The crystals grew as needles, with unit-cell parameters a = b = 84.82, c = 157.3 Å, α = β = 90, γ = 120°. LytR(N) crystallized in space group P6122 and the crystals diffracted to a maximum resolution of 2.34 Å. Based on the Matthews coefficient (V(M) = 5.44 Å(3) Da(-1)), one molecule is estimated to be present in the asymmetric unit.
应答调节蛋白LytR属于一类转录因子家族，参与调控病原菌中重要的毒力因子。该蛋白由一个接收结构域和一个效应结构域组成，它们在可控细胞死亡和裂解中发挥重要作用。LytR接收结构域（LytR(N)）已通过坐滴和悬滴气相扩散法进行了过量表达、纯化和结晶。晶体呈针状生长，晶胞参数为a = b = 84.82，c = 157.3 Å，α = β = 90，γ = 120°。LytR(N)在空间群P6122中结晶，晶体衍射到的最大分辨率为2.34 Å。根据马修斯系数（V(M) = 5.44 Å(3) Da(-1)），估计不对称单元中存在一个分子。
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