MurA作为土槿皮苷B和6-土槿皮苷B的主要作用靶点。

MurA as a primary target of tulipalin B and 6-tuliposide B.

作者信息

Shigetomi Kengo, Olesen Sanne Harder, Yang Yan, Mitsuhashi Shinya, Schönbrunn Ernst, Ubukata Makoto

机构信息

Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University.

出版信息

Biosci Biotechnol Biochem. 2013;77(12):2517-9. doi: 10.1271/bbb.130663. Epub 2013 Dec 7.

DOI:10.1271/bbb.130663

PMID:24317075

Abstract

(-)-Tulipalin B and (+)-6-tuliposide B were confirmed to inhibit MurA in vitro. However, contrary to fosfomycin, these compounds showed potent inhibitory activities against MurA overexpressing Escherichia coli, especially in the presence of UDP-GlcNAc. These observations suggest that these compounds induced bacterial cell death not through a MurA malfunction, but in such a MurA-mediated indirect manner as the inhibition of other Mur enzymes.

摘要

（-）-郁金香素B和（+）-6-郁金香苷B在体外被证实可抑制MurA。然而，与磷霉素相反，这些化合物对过表达MurA的大肠杆菌显示出强大的抑制活性，尤其是在存在UDP-GlcNAc的情况下。这些观察结果表明，这些化合物诱导细菌细胞死亡不是通过MurA功能障碍，而是以MurA介导的间接方式，如抑制其他Mur酶。