对附着于胶原蛋白的分离肝细胞的细胞膜电位进行微电极测量。

Microelectrode measurement of cell membrane potential in isolated hepatocytes attached to collagen.

作者信息

Petzinger E, Bigalke H

出版信息

Biochim Biophys Acta. 1986 Dec 16;863(2):318-24. doi: 10.1016/0005-2736(86)90274-9.

Abstract

Isolated spherical rat hepatocytes attached to collagen-coated cover slips generate a mean membrane potential (Em) of -78 +/- 9 mV as measured with high-resistance microelectrodes. The recordings were biphasic and were stable for upto 20 minutes. The correlation between external potassium concentration and Em was not linear. Several potassium-channel blockers did not effect the membrane potential. Addition of ouabain added to the incubation solution slowly depolarized the cells. The results indicate a high potassium permeability of the isolated spherical hepatocytes attached to collagen.

摘要

附着在胶原包被盖玻片上的分离球形大鼠肝细胞，用高电阻微电极测量时产生的平均膜电位（Em）为-78±9mV。记录是双相的，并且稳定长达20分钟。外部钾离子浓度与Em之间的相关性不是线性的。几种钾通道阻滞剂对膜电位没有影响。向孵育溶液中添加哇巴因会使细胞缓慢去极化。结果表明，附着在胶原上的分离球形肝细胞具有高钾通透性。