Universitat Jaume I, Departamento de Química Inorgánica y Orgánica, Av. Sos Baynat, s/n, E-12071 Castelló de la Plana, Spain.
Org Biomol Chem. 2014 Feb 7;12(5):823-31. doi: 10.1039/c3ob41773e. Epub 2013 Dec 9.
A new family of acidity sensitive fluorescent macrocycles has been synthesized and fully characterized. Their photophysical properties including emission quantum yield and fluorescence lifetime have been determined. The acid-base properties of the new molecules can be tuned by the incorporation of pendant functional groups. The nature of such functional groups (carboxylic acid or ester) influences dramatically the pKa of the probes, two compounds of which exhibit low values. Preliminary intracellular studies using confocal microscopy together with emission spectra of the probes from the cellular environment have shown that the synthesized fluorescent macrocycles mark the acidic organelles of RAW 264.7 macrophage cells.
已合成并充分表征了一种新的酸度敏感荧光大环家族。测定了它们的光物理性质，包括发射量子产率和荧光寿命。通过引入悬垂官能团可以调节新分子的酸碱性质。这些官能团的性质（羧酸或酯）强烈影响探针的 pKa，其中两种化合物具有较低的值。使用共聚焦显微镜和探针从细胞环境中的发射光谱进行的初步细胞内研究表明，合成的荧光大环标记 RAW 264.7 巨噬细胞的酸性细胞器。
