Kensova Renata, Blazkova Iva, Konecna Marie, Kopel Pavel, Chudobova Dagmar, Zitka Ondrej, Vaculovicova Marketa, Hynek David, Adam Vojtech, Beklova Miroslava, Kizek Rene
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic.
Int J Environ Res Public Health. 2013 Dec 2;10(12):6687-700. doi: 10.3390/ijerph10126687.
The aim of the study was the preparation of a liposome complex with encapsulated lead ions, which were electrochemically detected. In particular, experiments were focused on the potential of using an electrochemical method for the determination of free and liposome-encapsulated lead and determination of the encapsulation efficiency preventing the lead toxicity. Primarily, encapsulation of lead ions in liposomes and confirmation of successful encapsulation by electrochemical methods was done. Further, the reduction effect of the liposome matrix on the detected electrochemical signal was monitored. Besides encapsulation itself, comparison of toxicity of free lead ions and lead ions encapsulated in liposome was tested. The calculated IC50 values for evaluating the lead cytotoxicity showed significant differences between the lead enclosed in liposomes (28 µM) and free lead ions (237 µM). From the cytotoxicity studies on the bacterial strain of S. aureus it was observed that the free lead ions are less toxic in comparison with lead encapsulated in liposomes. Liposomes appear to be a suitable carrier of various substances through the inner cavity. Due to the liposome structure the lead enclosed in the liposome is more easily accepted into the cell structure and the toxicity of the enclosed lead is higher in comparison to free lead ions.
该研究的目的是制备一种包封有铅离子的脂质体复合物，并对其进行电化学检测。具体而言，实验重点在于利用电化学方法测定游离态和脂质体包封态铅的潜力，以及测定防止铅毒性的包封效率。首先，将铅离子包封于脂质体中，并通过电化学方法确认包封成功。此外，监测脂质体基质对检测到的电化学信号的还原作用。除了包封本身，还测试了游离铅离子与脂质体包封铅离子的毒性差异。计算得出的用于评估铅细胞毒性的IC50值显示，脂质体包封的铅（28 μM）与游离铅离子（237 μM）之间存在显著差异。从对金黄色葡萄球菌菌株的细胞毒性研究中观察到，与脂质体包封的铅相比，游离铅离子的毒性较小。脂质体似乎是各种物质通过内腔的合适载体。由于脂质体结构，脂质体包封的铅更容易进入细胞结构，并且与游离铅离子相比，包封铅的毒性更高。
