Chudobova Dagmar, Nejdl Lukas, Gumulec Jaromir, Krystofova Olga, Rodrigo Miguel Angel Merlos, Kynicky Jindrich, Ruttkay-Nedecky Branislav, Kopel Pavel, Babula Petr, Adam Vojtech, Kizek Rene
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
Int J Mol Sci. 2013 Jun 28;14(7):13592-614. doi: 10.3390/ijms140713592.
Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significant disadvantage of polymer products due to possible and very common colonization of the surface by various microorganisms that can pose a potential danger to the patient. One of the possible solutions is to prepare polymer with antibacterial/antimicrobial properties that is resistant to bacterial colonization. The aim of this study was to contribute to the development of antimicrobial polymeric material ideal for covering vascular implants with subsequent use in transplant surgery. Therefore, the complexes of polymeric substances (hyaluronic acid and chitosan) with silver nitrate or silver phosphate nanoparticles were created, and their effects on gram-positive bacterial culture of Staphylococcus aureus were monitored. Stages of formation of complexes of silver nitrate and silver phosphate nanoparticles with polymeric compounds were characterized using electrochemical and spectrophotometric methods. Furthermore, the antimicrobial activity of complexes was determined using the methods of determination of growth curves and zones of inhibition. The results of this study revealed that the complex of chitosan, with silver phosphate nanoparticles, was the most suitable in order to have an antibacterial effect on bacterial culture of Staphylococcus aureus. Formation of this complex was under way at low concentrations of chitosan. The results of electrochemical determination corresponded with the results of spectrophotometric methods and verified good interaction and formation of the complex. The complex has an outstanding antibacterial effect and this effect was of several orders higher compared to other investigated complexes.
由于聚合物具有良好的物理和化学性质，且具有经济优势，目前被广泛用于替代各种天然材料。聚合物应用的最重要分支之一是生产各种医疗用品。在这种情况下，由于聚合物产品表面可能被各种微生物大量定植，而这些微生物可能对患者构成潜在危险，因此有必要面对聚合物产品的这一重大缺点。一种可能的解决方案是制备具有抗菌/抗微生物特性且能抵抗细菌定植的聚合物。本研究的目的是为开发一种理想的抗菌聚合物材料做出贡献，该材料可用于覆盖血管植入物，随后用于移植手术。因此，制备了聚合物物质（透明质酸和壳聚糖）与硝酸银或磷酸银纳米颗粒的复合物，并监测了它们对金黄色葡萄球菌革兰氏阳性菌培养物的影响。使用电化学和分光光度法对硝酸银和磷酸银纳米颗粒与聚合物化合物形成复合物的阶段进行了表征。此外，使用生长曲线测定法和抑菌圈法测定了复合物的抗菌活性。本研究结果表明，壳聚糖与磷酸银纳米颗粒的复合物对金黄色葡萄球菌培养物具有抗菌作用，是最合适的。这种复合物在低浓度壳聚糖下即可形成。电化学测定结果与分光光度法结果一致，验证了复合物的良好相互作用和形成。该复合物具有出色的抗菌效果，与其他研究的复合物相比，这种效果高出几个数量级。
