Botany Department, University of Washington, 98195, Seattle, WA, USA.
Planta. 1979 Jan;145(5):437-42. doi: 10.1007/BF00380097.
We have compared the effects of cycloheximide (CHI) and two other rapid and effective inhibitors of protein synthesis, pactamycin and 2-(4-methyl-2,6-dinitroanilino)-N-methyl proprionamide (MDMP), on protein synthesis, respiration, auxin-induced growth and H(+)-excreation of Avena sativa L. coleoptiles. All three compounds inhibit protein synthesis without affecting respiration. The effectiveness of the inhibitors against H(+)-excretion and growth correlates with their ability to inhibit protein synthesis. Both CHI and MDMP inhibit auxin-induced H(+)-excretion after a latent period of 5-8 min, and inhibit growth after a 8-10-min lag. These results support the idea that continued protein synthesis is required in the initial stages of the growth-promoting action of auxin.
我们比较了环己亚胺（CHI）和另外两种快速有效的蛋白质合成抑制剂——放线菌酮和 2-(4-甲基-2,6-二硝基苯胺基)-N-甲基丙酰胺（MDMP）对蛋白质合成、呼吸作用、生长素诱导的生长和 H(+)分泌的影响。这三种化合物都能抑制蛋白质合成而不影响呼吸作用。抑制剂对 H(+)分泌和生长的抑制作用与它们抑制蛋白质合成的能力相关。CHI 和 MDMP 在潜伏期 5-8 分钟后抑制生长素诱导的 H(+)分泌，在 8-10 分钟的滞后后抑制生长。这些结果支持这样一种观点，即生长素促进生长作用的初始阶段需要持续的蛋白质合成。
