White M C, Newland P, Daniels M, Turner S J, Mathias D, Teasdale G, Kendall-Taylor P
Clin Endocrinol (Oxf). 1986 Aug;25(2):173-9. doi: 10.1111/j.1365-2265.1986.tb01679.x.
Cell culture methods were used to assess whether human pituitary adenomas secreting GH and associated with clinical acromegaly also secreted the structurally unrelated glycoprotein hormone alpha-subunit. Thirty-two tumours, together with peri-adenomatous tissue from two of them and three normal pituitaries were studied. Anterior pituitary hormones were measured by radioimmunoassay and included PRL, TSH, LH, FSH, and ACTH, as well as GH and alpha-subunit. Normal pituitary tissues secreted all hormones assayed. All 32 tumours secreted GH ranging from 241 to 5556 ng/2 X 10(5) cells/24 h and 12 (37.5%) secreted alpha-subunit in amounts which could not be accounted for by cross-reaction of other hormones or contamination by normal pituitary tissue, and which ranged from 10.3 to 73.5 ng/2 X 10(5) cells/24 h. Ten other tumours also secreted alpha-subunit but in very small amounts, not exceeding 1.8 ng/2 X 10(5) cells/24 h. PRL was secreted from 21 tumours (66%), and small amounts of other hormones, chiefly LH and TSH, were occasionally secreted from tumours. These cell culture studies would suggest that pituitary adenomas causing acromegaly are hormonally heterogeneous and that PRL and glycoprotein alpha-subunit are commonly detected in addition to GH.
采用细胞培养方法评估分泌生长激素（GH）并伴有临床肢端肥大症的人垂体腺瘤是否也分泌结构不相关的糖蛋白激素α亚基。研究了32个肿瘤，以及其中2个肿瘤的腺瘤周围组织和3个正常垂体。通过放射免疫测定法测量垂体前叶激素，包括催乳素（PRL）、促甲状腺激素（TSH）、促黄体生成素（LH）、促卵泡激素（FSH）和促肾上腺皮质激素（ACTH），以及GH和α亚基。正常垂体组织分泌所有检测的激素。所有32个肿瘤均分泌GH，范围为241至5556 ng/2×10⁵细胞/24小时，12个（37.5%）肿瘤分泌α亚基，其分泌量不能用其他激素的交叉反应或正常垂体组织污染来解释，范围为10.3至73.5 ng/2×10⁵细胞/24小时。另外10个肿瘤也分泌α亚基，但量非常少，不超过1.8 ng/2×10⁵细胞/24小时。21个肿瘤（66%）分泌PRL，肿瘤偶尔分泌少量其他激素，主要是LH和TSH。这些细胞培养研究表明，导致肢端肥大症的垂体腺瘤在激素方面具有异质性，除GH外，通常还可检测到PRL和糖蛋白α亚基。
