Department of Biological Sciences, Washington-Singer Laboratories, University of Exeter, EX4 4QG, Exeter, Devon, U.K..
Planta. 1979 Oct;146(5):591-6. doi: 10.1007/BF00388837.
Levels of free-abscisic acid and "boundabscisic acid" (alkaline hydrolyzable abscisic acidesters) in replicated samples of terminal vegetative buds of sycamore trees were measured during natural emergence from winter dormancy by gas chromatographic methods together with isotope dilution estimation of recovery rates. Not until after the buds had been released from true dormancy in January by winter chilling did any clear change occur in either abscisic acid (ABA) fraction, or in total ABA, on any basis of comparison. The percentage of total ABA present as the free acid declined at the end of true dormancy to approximately two-thirds of its value in the earlier winter months. It is concluded that glucosylation of ABA is unlikely to play a major part in the mechanism of release from dormancy in vegetative sycamore buds. At the end of true dormancy there was a large transient increase in what appeared to be phaseic acid, but this was not accompanied by any marked decrease in either free- or bound-ABA.
在通过气相色谱法测量自然解除冬季休眠的过程中，我们对 replicated samples of terminal vegetative buds of sycamore trees 中的游离脱落酸和“结合态脱落酸”（碱性水解脱落酸酯）的水平进行了测量，并通过同位素稀释法估计了回收率。直到 1 月冬季寒冷使芽真正休眠解除后，游离脱落酸（ABA）或任何比较基础上的总 ABA 才发生任何明显变化。在真正休眠结束时，总 ABA 中作为游离酸存在的比例下降到其在冬季前几个月值的三分之二左右。因此，我们得出结论，ABA 的葡萄糖基化不太可能在营养性休眠解除机制中起主要作用。在真正休眠结束时，出现了大量的相位酸的短暂增加，但这并没有伴随着游离或结合态 ABA 的任何明显减少。
