Division of Plant Industry, CSIRO, P.O. Box 1600, 2601, Canberra, Australia.
Plant Mol Biol. 1983 Sep;2(5):259-67. doi: 10.1007/BF01578644.
Serological studies and comparison of N-terminal amino acid sequences with the amino acid sequence deduced from a cDNA clone have been used to establish the sequence relationships between the subunits of the pea seed storage protein, vicilin. Subunits smaller than Mr∼50 000 (i.e., Mr 34 000, 30 000, 25 000, 18 000, 14 000, 13 000 and 12 000) show extensive homology with molecules within Mr∼50 000 group. Both the sequencing and serological data confirm earlier evidence from studies on vicilin synthesisin vivo andin vitro which indicated that the vicilin subunits smaller than Mr∼50 000 arose by endoproteolytic cleavage of parent molecules within the Mr∼50 000 group. Cleavage in different Mr 50 000 parent molecules containing either one or both of two susceptible processing sites accounts for the formation of all the vicilin subunits smaller than Mr∼50 000, with the possible exception of the Mr34 000 polypeptide. The position of these sites in the putative parents were defined by reference to a complete amino acid sequence deduced from the sequence of DNA complementary to mRNA for one member of the Mr∼50 000 group.
血清学研究和与 cDNA 克隆推导的氨基酸序列的比较已被用于确定豌豆种子贮藏蛋白 vicilin 亚基之间的序列关系。小于 Mr∼50000（即 Mr 34000、30000、25000、18000、14000、13000 和 12000）的亚基与 Mr∼50000 组内的分子具有广泛的同源性。测序和血清学数据都证实了早先在体内和体外 vicilin 合成研究中获得的证据，这些证据表明，小于 Mr∼50000 的 vicilin 亚基是通过 Mr∼50000 组内的母体分子内的内切蛋白酶切割产生的。不同 Mr 50000 母体分子中的两个易处理位点中的一个或两个的切割导致了所有小于 Mr∼50000 的 vicilin 亚基的形成，可能除了 Mr34000 多肽外。这些位点在假定的母体中的位置是通过参考与 Mr∼50000 组中一个成员的 mRNA 互补的 DNA 序列推导的完整氨基酸序列来定义的。
