大鼠体内膳食蛋氨酸、铅和林丹的相互作用。

Interactions of dietary methionine, lead and lindane in rats.

作者信息

Rowe V A, Hathcock J N, Serfass R E, Shriver C N

出版信息

Drug Nutr Interact. 1986;4(4):349-54.

Abstract

The effects of dietary methionine on lead and lindane toxicities in rats were studied in two experiments. Rats were fed methionine-deficient (60% of requirement) or methionine-sufficient soy protein-based diets with lead acetate added (10,000 mg/kg Pb) and treated with a single dose of lindane (25% of LD50 or 88 mg/kg, p.o.) in both experiments. In experiment I, all rats were fed ad libitum. In experiment II, rats fed the methionine-sufficient diet were pair-fed to rats fed the methionine-deficient diet. In experiments I and II, the methionine-sufficient and the methionine-deficient rats had decreased final body weights, increased liver weights, decreased hematocrits, and no changes in glutathione S-transferase activity when compared to a control group. Lead + lindane treatments increased liver glutathione levels in the methionine-sufficient and methionine-deficient rats in both experiments. However, in experiment II (pair-feeding), the methionine-sufficient rats had a much greater level of liver glutathione than the methionine-deficient rats. The methionine status of the animals seems to be an important factor in determining the liver glutathione level of pair-fed rats treated with lead + lindane.

摘要

在两项实验中研究了膳食蛋氨酸对大鼠铅和林丹毒性的影响。在两项实验中，给大鼠喂食缺乏蛋氨酸（需求量的60%）或充足蛋氨酸的大豆蛋白基饮食，并添加醋酸铅（10,000 mg/kg铅），并用单剂量林丹（LD50的25%或88 mg/kg，口服）进行处理。在实验I中，所有大鼠自由采食。在实验II中，给喂食充足蛋氨酸饮食的大鼠与喂食缺乏蛋氨酸饮食的大鼠进行配对喂食。在实验I和II中，与对照组相比，充足蛋氨酸和缺乏蛋氨酸的大鼠最终体重降低、肝脏重量增加、血细胞比容降低，谷胱甘肽S-转移酶活性无变化。在两项实验中，铅+林丹处理均增加了充足蛋氨酸和缺乏蛋氨酸大鼠的肝脏谷胱甘肽水平。然而，在实验II（配对喂食）中，充足蛋氨酸的大鼠肝脏谷胱甘肽水平比缺乏蛋氨酸的大鼠高得多。动物的蛋氨酸状态似乎是决定铅+林丹处理的配对喂食大鼠肝脏谷胱甘肽水平的一个重要因素。