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编码环磷酸腺苷（cAMP）依赖性蛋白激酶II型调节亚基的cDNA的表达克隆。

Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP-dependent protein kinase.

作者信息

Hemmings B A, Schwarz M, Adavani S R, Jans D A

出版信息

FEBS Lett. 1986 Dec 15;209(2):219-22. doi: 10.1016/0014-5793(86)81114-0.

DOI:10.1016/0014-5793(86)81114-0
PMID:2431926
Abstract

We report here the isolation and sequence of a cDNA for the type II regulatory subunit of the cAMP-dependent protein kinase (cAMP-PK) from a lambda gt-11 cDNA library derived from a porcine epithelial cell line (LLC-PK1). The cDNA was detected by immunological screening using an affinity purified polyclonal antibody for bovine RII. DNA sequence analysis of the 467 bp EcoRI insert confirmed the identity of the clone, because the deduced amino acid sequence corresponded to the published sequence for the bovine RII protein. Northern analysis of total RNA from the LLC-PK1 cells indicated a single mRNA species of about 6.0 kb, probably derived from a single copy gene.

摘要

我们在此报告从猪上皮细胞系（LLC-PK1）来源的λgt-11 cDNA文库中分离出的cAMP依赖性蛋白激酶（cAMP-PK）II型调节亚基的cDNA及其序列。使用针对牛RII的亲和纯化多克隆抗体通过免疫筛选检测到该cDNA。对467 bp的EcoRI插入片段进行DNA序列分析证实了该克隆的身份，因为推导的氨基酸序列与已发表的牛RII蛋白序列相符。对LLC-PK1细胞的总RNA进行Northern分析表明，存在一种约6.0 kb的单一mRNA种类，可能来自单拷贝基因。

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Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP-dependent protein kinase.编码环磷酸腺苷（cAMP）依赖性蛋白激酶II型调节亚基的cDNA的表达克隆。
FEBS Lett. 1986 Dec 15;209(2):219-22. doi: 10.1016/0014-5793(86)81114-0.
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