Page T M, Jacobsen S J, Nyhan W L, Mangum J H, Robins R K
Int J Biochem. 1986;18(10):957-60. doi: 10.1016/0020-711x(86)90078-9.
The metabolism of the purine analogs 3-deazaguanine and 3-deazaguanosine was studied in cultured human cells using radiolabeled tracers, individual enzyme assays, and mutant cell lines. The toxicity of each drug appeared to require conversion to the 5' nucleotide. The base was converted to the nucleotide by hypoxanthine guanine phosphoribosyl transferase. The conversion of the nucleoside to the nucleotide was catalyzed by an unidentified kinase. Purine nucleoside 3-deazaguanosine-5'-monophosphate was converted to its corresponding di- and triphosphate by guanylate kinase. Both the base and the nucleoside were incorporated into DNA but not RNA.
利用放射性标记示踪剂、个体酶测定法和突变细胞系，在培养的人细胞中研究了嘌呤类似物3-脱氮鸟嘌呤和3-脱氮鸟苷的代谢。每种药物的毒性似乎都需要转化为5'核苷酸。碱基由次黄嘌呤鸟嘌呤磷酸核糖基转移酶转化为核苷酸。核苷向核苷酸的转化由一种未鉴定的激酶催化。嘌呤核苷3-脱氮鸟苷-5'-单磷酸由鸟苷酸激酶转化为其相应的二磷酸和三磷酸。碱基和核苷都掺入了DNA而非RNA。
