Department of Internal Medicine, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:753829. doi: 10.1155/2013/753829. Epub 2013 Nov 11.
Introduction. This study was designed to investigate the effects of low level laser therapy (LLLT) on experimental allergic rhinitis (AR) models induced by ovalbumin. Materials and Methods. AR was induced by 1% ovalbumin in mice. Twenty-four mice were divided into 4 groups: normal, control, low, and high dose irradiation. Low and high dose LLLT were irradiated once a day for 7 days. Total IgE, cytokines concentrations (IL-4 and IFN- γ ), and thymus and activation regulated chemokine (TARC) were measured. Histological changes in the nasal mucosal tissue by laser irradiation were examined. Results. LLLT significantly inhibited total IgE, IL-4, and TARC expression in ovalbumin-induced mice at low dose irradiation. The protein expression level of IL-4 in spleen was inhibited in low dose irradiation significantly. IL-4 expression in EL-4 cells was inhibited in a dose dependent manner. Histological damages of the epithelium in the nasal septum were improved by laser irradiation with marked improvement at low dose irradiation. Conclusion. These results suggest that LLLT might serve as a new therapeutic tool in the treatment of AR with more effectiveness at low dose irradiation. To determine the optimal dose of laser irradiation and action mechanisms of laser therapy, further studies will be needed.
简介。本研究旨在探讨低水平激光疗法（LLLT）对卵清蛋白诱导的实验性变应性鼻炎（AR）模型的影响。
材料与方法。AR 通过 1%卵清蛋白在小鼠中诱导。24 只小鼠被分为 4 组：正常、对照、低剂量和高剂量照射组。低剂量和高剂量 LLLT 每天照射一次，持续 7 天。测量总 IgE、细胞因子浓度（IL-4 和 IFN-γ）和胸腺激活调节趋化因子（TARC）。检查激光照射对鼻黏膜组织的组织学变化。
结果。低剂量照射时，LLLT 显著抑制卵清蛋白诱导的小鼠总 IgE、IL-4 和 TARC 的表达。低剂量照射时，脾内 IL-4 蛋白表达水平显著抑制。IL-4 在 EL-4 细胞中的表达呈剂量依赖性抑制。激光照射改善了鼻中隔上皮的组织学损伤，低剂量照射时明显改善。
结论。这些结果表明，LLLT 可能成为 AR 治疗的一种新的治疗工具，低剂量照射效果更好。为了确定激光照射的最佳剂量和激光治疗的作用机制，需要进一步研究。
