Bukharova T B, Logovskaya L V, Volkov A V, Garas M N, Vikhrova E B, Logunov D Yu, Makhnach O V, Shmarov M M, Gol'dshtein D V
Medical Genetic Research Center, Russian Academy of Medical Sciences; ReMeTeks Company; N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Bull Exp Biol Med. 2013 Nov;156(1):122-6. doi: 10.1007/s10517-013-2294-y.
We determined conditions for effective transduction of multipotent mesenchymal stromal cells from human adipose tissue with adenoviral constructs carrying the gene of human bone morphogenetic protein BMP-2. The peak of transgene transcription and BMP-2 protein secretion in the transduced cultures was observed on day 6 after infection. The maximum transcription of BMP-2 gene and genes of osteogenic markers (bone sialoprotein, osteopontin, and osteocalcin) was observed in the medium containing sodium β-glycerophosphate and ascorbic acid. Addition of D 3 vitamin did not enhance the expression of BMP-2 gene in transduced cells. The obtained cell cultures with high osteogenic potential can be used in bone tissue engineering.
我们确定了用携带人骨形态发生蛋白BMP-2基因的腺病毒构建体有效转导人脂肪组织多能间充质基质细胞的条件。在感染后第6天观察到转导培养物中转基因转录和BMP-2蛋白分泌的峰值。在含有β-甘油磷酸钠和抗坏血酸的培养基中观察到BMP-2基因和成骨标志物（骨唾液蛋白、骨桥蛋白和骨钙素）基因的最大转录。添加维生素D3并未增强转导细胞中BMP-2基因的表达。所获得的具有高成骨潜力的细胞培养物可用于骨组织工程。
