Savchik E Yu, Kalinina T B, Drozd N N, Makarov V A, Zav'yalova E G, Lapsheva E N, Mudrik N N, Babij A V, Pavlova G V, Golovin A V, Kopylov A M
Hematology Research Center, Ministry of Health and Social Development of the Russian Federation; APTO-PHARM, Moscow Region; Chemical Faculty, Faculty of Bioengineering and Bioinformatics, M. V. Lomonosov Moscow State University; Institute of Gene Biology, Russian Academy of Sciences, Moscow.
Bull Exp Biol Med. 2013 Nov;156(1):44-8. doi: 10.1007/s10517-013-2274-2.
RA36 DNA aptamer is a direct anticoagulant prolonging clotting time of human, rabbit, and rat plasma in the thrombin time test. Anticoagulant activity of RA36 is lower than that of recombinant hirudin. During inhibition of human plasma clotting activated with echitox (coagulase from Echis multisquamatus venom), the aptamer presumably binds to meisothrombin exosite I. The sensitivity of human plasma to the aptamer 5-fold surpasses that of rat plasma. Analysis of RA36 binding to coagulase of Agkistrodon halys venom (ancistron) is required for proving the effect of aptamer on polymerization of human fibrinogen.
RA36 DNA适配体是一种直接抗凝剂，在凝血酶时间试验中可延长人、兔和大鼠血浆的凝血时间。RA36的抗凝活性低于重组水蛭素。在用锯鳞蝰蛇毒素（来自多鳞蝰蛇毒液的凝固酶）激活人血浆凝血过程中，该适配体可能与中凝血酶的外位点I结合。人血浆对该适配体的敏感性比大鼠血浆高5倍。为了证明适配体对人纤维蛋白原聚合的影响，需要分析RA36与蝮蛇毒液凝固酶（Ancistron）的结合情况。
