人乳头瘤病毒16型E6和E7的血清阳性与口腔癌风险

Seropositivity of HPV 16 E6 and E7 and the risk of oral cancer.

作者信息

Wong G R, Ha K O, Himratul-Aznita W H, Yang Y H, Wan Mustafa W M, Yuen K M, Abraham M T, Tay K K, Karen-Ng L P, Cheong S C, Zain R B

机构信息

Oral Cancer Research & Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia; Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.

出版信息

Oral Dis. 2014 Nov;20(8):762-7. doi: 10.1111/odi.12218. Epub 2014 Jan 21.

DOI:10.1111/odi.12218

PMID:24320099

Abstract

OBJECTIVES

The objective of the study was to determine the prevalence of HPV seropositivity among patients with oral squamous cell carcinoma (OSCC) and healthy individuals and to correlate the association between HPV 16 seropositivity and risk of OSCC.

MATERIALS AND METHODS

HPV 16 E6 and E7 plasmids were constructed for the production of recombinant protein, which was used as the antigen in ELISA. HPV ELISA was performed on serum samples from 50 healthy individuals and 50 patients with OSCC.

RESULTS

Using the HPV ELISA, 30% (OR = 2.25, 95% CI = 0.85-5.93) and 18% (OR = 1.61, 95% CI = 0.53-4.92) of patients with oral cancer were found to be HPV 16 E6 and E7 seropositive, respectively. Significant association was found between HPV 16 seropositivity and increased risk of OSCC in men, but not in male subjects. A similar trend was observed in non-betel quid chewers.

CONCLUSIONS

Potential associations between HPV 16 E6/E7 seropositivity and oral cancer were revealed in men and non-betel quid chewer subjects, suggesting a possible etiological role of HPV 16 in subgroup of patients with OSCC in Malaysia.

摘要

目的

本研究的目的是确定口腔鳞状细胞癌（OSCC）患者和健康个体中HPV血清阳性的患病率，并关联HPV 16血清阳性与OSCC风险之间的关系。

材料与方法

构建HPV 16 E6和E7质粒以生产重组蛋白，该重组蛋白用作ELISA中的抗原。对50名健康个体和50名OSCC患者的血清样本进行HPV ELISA检测。

结果

使用HPV ELISA检测发现，口腔癌患者中分别有30%（OR = 2.25，95%CI = 0.85 - 5.93）和18%（OR = 1.61，95%CI = 0.53 - 4.92）的患者HPV 16 E6和E7血清呈阳性。在男性中发现HPV 16血清阳性与OSCC风险增加之间存在显著关联，但在男性受试者中未发现。在非嚼槟榔者中观察到类似趋势。