Hei T K, Hall E J, Kushner S, Osmak R S
Int J Hyperthermia. 1986 Jul-Sep;2(3):311-20. doi: 10.3109/02656738609016488.
The modulating effects of hyperthermia on cytotoxity and oncogenicity of several chemotherapeutic agents were investigated using the C3H 10T1/2 cell system. Logarithmic phase cultures of 10T1/2 cells were treated with various doses of cis-platinum or bleomycin sulphate for 2 h, either alone or with simultaneous hyperthermia (42 degrees C for 2 h). In a second set of experiments, cells were treated for 24 h with either melphalan or cis-platinum followed by a 2 h heat treatment. While hyperthermia alone was found to be ineffective in inducing oncogenic transformation and was only moderately cytotoxic, concurrent hyperthermia with drug treatment enhanced both the toxicity and oncogenic transforming potential of drugs 3-4-fold in C3H 10T1/2 cells. Sequential heat after drug treatment, however, was found to increase toxicity slightly but had no effect on the oncogenicity of the drugs.
利用C3H 10T1/2细胞系统研究了热疗对几种化疗药物细胞毒性和致癌性的调节作用。对数期培养的10T1/2细胞分别用不同剂量的顺铂或硫酸博来霉素处理2小时，处理方式为单独处理或同时进行热疗（42℃，2小时）。在另一组实验中，细胞先用美法仑或顺铂处理24小时，然后进行2小时的热处理。虽然发现单独热疗在诱导致癌转化方面无效，且仅有中度细胞毒性，但热疗与药物治疗同时进行时，可使C3H 10T1/2细胞中药物的毒性和致癌转化潜能增强3至4倍。然而，药物处理后进行序贯热疗，发现毒性略有增加，但对药物的致癌性没有影响。
