Hong Ki-Sung, Byun Ki-Hyun, Seo Joseph, Lee Hye-jin, Choi Jong-jin, Kim Kyung Sul, Choi Youngsok, Moon Sung-Hwan, Chung Hyung-Min
Stem Cell Research Laboratory, Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Seoul, Republic of Korea.
Biotechnol Lett. 2014 Apr;36(4):859-68. doi: 10.1007/s10529-013-1414-0. Epub 2013 Dec 10.
Evaluation of therapeutic effects of transplanted cells in ischemic heart failure models are important issues. However, traditional injection needles that are widely used in clinical practice tend to reduce the amount of functional cells relative to the injected amount. We now describe a cell transplantation technique using a screw needle. After inducing acute myocardial infarction in a rat model, human embryonic stem cell-derived endothelial cells were injected into the infarcted regions with a screw or straight-curved needle. When an equal volume of cells was transplanted, the screw group suffered minimal cell loss, showed improvement in LV wall thickness (74.5 ± 6.2 vs. 64.4 ± 7.8 %), epicardium scar length (19.3 ± 2.8 vs. 24.6 ± 6.4 %), and area of engraft. Thus, even a simple change in the structure of an instrument can have a large impact on transplantation efficiency.
评估移植细胞在缺血性心力衰竭模型中的治疗效果是重要课题。然而，临床实践中广泛使用的传统注射针往往会使功能细胞数量相对于注射量减少。我们现在描述一种使用螺旋针的细胞移植技术。在大鼠模型中诱导急性心肌梗死后，将人胚胎干细胞衍生的内皮细胞用螺旋针或直弯针注入梗死区域。当移植等量细胞时，螺旋针组细胞损失最小，左心室壁厚度有所改善（74.5±6.2%对64.4±7.8%），心外膜瘢痕长度（19.3±2.8对24.6±6.4%）以及植入面积也有所改善。因此，即使仪器结构的一个简单改变也可能对移植效率产生很大影响。
