Nagai M, Abe T, Seki S, Minato K, Watanabe S, Shimoyama M
Jpn J Clin Oncol. 1986 Dec;16(4):397-406.
Fresh tumor cells from pleural effusion of a patient with Hodgkin's disease were analyzed cytogenetically, immunologically and enzymocytochemically. They were characterized by the presence of alpha-naphthyl butyrate esterase activity, Fc gamma-receptor, HLA-DR antigen and No. 9 antigen which has been shown to be present in Hodgkin's cells and granulocytes, and the absence of definite T-, B- and myeloid cell markers. The karyotype analysis of these tumor cells revealed chromosome instability, but the clonality was confirmed by the many common abnormalities such as -4, -6, -10, -12, -13, -14, +21, del(X) (q22,q26), del(7) (q32q36), and +der(19)t(19;?). In addition, there were more duplicated tetraploid clones than near-diploid clones. The karyotype of the near-diploid clone was interpreted as: 48, X, del(X) (q22q26), -4, -6, -10, -12, -13, -14, +20, +21, +der(4)t(4;?) (p16;?), del(7) (q32q36), +der(19)t(19;?) (p13;?), +mar1, +mar2, +mar3. The karyotype abnormalities characteristic of lymphomas or leukemias were not found. These results indicate that the tumor cells are not of lymphoid or myeloid lineage. Further studies are needed to determine the cellular origin of the tumor cells of Hodgkin's disease.
对一名霍奇金病患者胸腔积液中的新鲜肿瘤细胞进行了细胞遗传学、免疫学和酶细胞化学分析。这些细胞的特征是存在α-萘丁酸酯酶活性、Fcγ受体、HLA-DR抗原以及已证实在霍奇金细胞和粒细胞中存在的9号抗原，并且缺乏明确的T、B和髓细胞标志物。对这些肿瘤细胞的核型分析显示染色体不稳定，但通过许多常见异常，如-4、-6、-10、-12、-13、-14、+21、del(X)(q22,q26)、del(7)(q32q36)和+der(19)t(19;?)证实了克隆性。此外，四倍体重复克隆比近二倍体克隆更多。近二倍体克隆的核型解释为：48,X,del(X)(q22q26),-4,-6,-10,-12,-13,-14,+20,+21,+der(4)t(4;?)(p16;?),del(7)(q32q36),+der(19)t(19;?)(p13;?),+mar1,+mar2,+mar3。未发现淋巴瘤或白血病特有的核型异常。这些结果表明肿瘤细胞不是淋巴样或髓样谱系。需要进一步研究以确定霍奇金病肿瘤细胞的细胞起源。
