小鼠海马体中半乳糖凝集素-1阳性细胞的特征分析。

Characterization of galectin-1-positive cells in the mouse hippocampus.

作者信息

Kajitani Kosuke, Kobayakawa Yuko, Nomaru Hiroko, Kadoya Toshihiko, Horie Hidenori, Nakabeppu Yusaku

机构信息

aDivision of Neurofunctional Genomics, Medical Institute of Bioregulation bDepartment of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka cDepartment of Biotechnology, Maebashi Institute of Technology, Maebashi, Gunma dTechnoMaster Co. Ltd, Yokohama, Japan.

出版信息

Neuroreport. 2014 Feb 12;25(3):171-6. doi: 10.1097/WNR.0000000000000068.

DOI:10.1097/WNR.0000000000000068

PMID:24323124

Abstract

Galectin-1 (gal-1) is one of several well-studied proteins from the galectin families. It is a 14.5 kDa glycoprotein with a single carbohydrate-binding domain. To examine the distribution and properties of gal-1 in the mouse hippocampus, we performed immunohistochemistry using an anti-gal-1 antibody. We found that most gal-1-positive cells showed both NeuN and β-tubulin III (Tuj-1) immunoreactivity (NeuN: 93%, β-tubulin III: 88%). Furthermore, we clarified that 77% of gal-1-positive cells expressed somatostatin, 79% of gal-1-positive cells expressed GAD67, 34% of gal-1-positive cells expressed parvalbumin, 5% of gal-1-positive cells expressed calretinin, 2% of gal-1-positive cells expressed calbindin, and 31% of gal-1-positive cells expressed neuropeptide Y in the mouse hippocampus. These results indicate that gal-1 is expressed in interneurons that also express β-tubulin III and gal-1 may be a novel marker for interneuron subpopulations in the hippocampus.

摘要

半乳糖凝集素-1（gal-1）是半乳糖凝集素家族中几种经过充分研究的蛋白质之一。它是一种14.5 kDa的糖蛋白，具有一个碳水化合物结合结构域。为了研究gal-1在小鼠海马体中的分布和特性，我们使用抗gal-1抗体进行了免疫组织化学实验。我们发现，大多数gal-1阳性细胞同时显示NeuN和β-微管蛋白III（Tuj-1）免疫反应性（NeuN：93%，β-微管蛋白III：88%）。此外，我们还明确了在小鼠海马体中，77%的gal-1阳性细胞表达生长抑素，79%的gal-1阳性细胞表达GAD67，34%的gal-1阳性细胞表达小白蛋白，5%的gal-1阳性细胞表达钙视网膜蛋白，2%的gal-1阳性细胞表达钙结合蛋白，31%的gal-1阳性细胞表达神经肽Y。这些结果表明，gal-1在同时表达β-微管蛋白III的中间神经元中表达，并且gal-1可能是海马体中间神经元亚群的一种新型标志物。