Glover J C, Petursdottir G, Jansen J K
J Neurosci Methods. 1986 Nov;18(3):243-54. doi: 10.1016/0165-0270(86)90011-7.
We have used recently developed fluorescein- and rhodamine-conjugated dextran-amines as axonal tracers in in vitro preparations of the nervous system of the chicken embryo. These substances are efficiently taken up by injured axons and transported rapidly to the cell bodies. They are also, albeit to a lesser degree, taken up by intact nerve terminals in skeletal muscle. They reveal the dendritic and axonal structure of labelled neurons, and are well suited for use in double-labelling experiments.
我们最近使用了新开发的荧光素和罗丹明共轭葡聚糖胺作为鸡胚神经系统体外制剂中的轴突示踪剂。这些物质能被受损轴突有效摄取，并迅速运输到细胞体。它们也会被骨骼肌中的完整神经末梢摄取，尽管程度较轻。它们能揭示标记神经元的树突和轴突结构，非常适合用于双重标记实验。
