Catsicas S, Berbel P J, Innocenti G M
J Neurosci Methods. 1986 Dec;18(4):325-32. doi: 10.1016/0165-0270(86)90020-8.
Central nervous system structures containing neurons labeled by the fluorescent tracers Fast blue (FB), Diamidino yellow dihydrochloride (DY), Rhodamine B isothiocyanate (RITC) and Rhodamine-labeled latex microspheres (RLM) were processed with the Golgi method. The goal was to improve the visualization of the fluorescent labeled neurons and to allow their ultrastructural examination. While the fluorescence of FB and RITC is greatly attenuated by the Golgi method, RLM and DY are still visible in Golgi-impregnated neurons. However, it is usually necessary to remove the silver precipitate by gold-toning.
含有经荧光示踪剂快蓝（FB）、二盐酸双脒基黄（DY）、异硫氰酸罗丹明B（RITC）和罗丹明标记乳胶微球（RLM）标记的神经元的中枢神经系统结构，采用高尔基方法进行处理。目的是改善荧光标记神经元的可视化效果，并对其进行超微结构检查。虽然高尔基方法会使FB和RITC的荧光大大减弱，但在经高尔基浸染的神经元中仍可看到RLM和DY。然而，通常需要通过金调色法去除银沉淀。
