Department of Radiology, Philipps University Marburg, Germany.
Anticancer Res. 2013 Dec;33(12):5213-21.
The aim of this study was to evaluate microarray technology in the detection of micrometastases of head and neck squamous cell carcinoma (HNSCC) in muscle tissue. Three hundred SCCVII tumor cells were injected intramuscularly into the right flank of ten C3H/Km mice. One week later, the animals were euthanized and the muscle tissue was taken out. Histology (H&E staining), microarray and reverse transcriptase polymerase chain reaction analysis (RT-PCR) of the tissue was performed. Histology showed a few tumor cells between the muscle fibers. Microarray technology showed the different gene expression pattern of the muscle tissue with micrometastases in comparison to normal muscle tissue. Only genes with a fold change difference of 10 or greater were considered. Gene expression analysis revealed changes in the expression levels of 91 genes of micrometastases in muscle tissue. RT-PCR confirmed gene up-regulation. Significant differences in gene expression between micrometastases in muscle tissue and pure muscle tissue were found. The genes found to be up-regulated could be used to detect micrometastases in muscle tissue.
本研究旨在评估微阵列技术在检测头颈部鳞状细胞癌（HNSCC）肌肉组织微转移中的应用。将 300 个 SCCVII 肿瘤细胞注射到 10 只 C3H/Km 小鼠的右侧肋部。1 周后，处死动物并取出肌肉组织。对组织进行组织学（H&E 染色）、微阵列和逆转录聚合酶链反应分析（RT-PCR）。组织学显示少数肿瘤细胞位于肌肉纤维之间。微阵列技术显示，与正常肌肉组织相比，有微转移的肌肉组织具有不同的基因表达模式。只有差异倍数为 10 或更大的基因才被认为是有差异的。基因表达分析显示，肌肉组织微转移中有 91 个基因的表达水平发生了变化。RT-PCR 证实了基因的上调。在肌肉组织微转移和纯肌肉组织之间发现了基因表达的显著差异。上调的基因可用于检测肌肉组织中的微转移。
