College of Agronomy, Northwest Agriculture & Forestry University, Yangling, Shaanxi, China ; Department of Agronomy, Iowa State University, Ames, Iowa, United States of America.
PLoS One. 2013 Dec 6;8(12):e82333. doi: 10.1371/journal.pone.0082333. eCollection 2013.
Aerial plant surfaces are covered by epicuticular waxes that among other purposes serve to control water loss. Maize glossy mutants originally identified by their "glossy" phenotypes exhibit alterations in the accumulation of epicuticular waxes. By combining data from a BSR-Seq experiment and the newly developed Seq-Walking technology, GRMZM2G118243 was identified as a strong candidate for being the glossy13 gene. The finding that multiple EMS-induced alleles contain premature stop codons in GRMZM2G118243, and the one knockout allele of gl13, validates the hypothesis that gene GRMZM2G118243 is gl13. Consistent with this, GRMZM2G118243 is an ortholog of AtABCG32 (Arabidopsis thaliana), HvABCG31 (barley) and OsABCG31 (rice), which encode ABCG subfamily transporters involved in the trans-membrane transport of various secondary metabolites. We therefore hypothesize that gl13 is involved in the transport of epicuticular waxes onto the surfaces of seedling leaves.
植株气生表面被角质层蜡质覆盖，其具有控制水分流失等作用。最初因其“光亮”表型而被鉴定为玉米光亮突变体，表现出角质层蜡质积累的改变。通过将 BSR-Seq 实验和新开发的 Seq-Walking 技术的数据相结合，鉴定到 GRMZM2G118243 是光亮 13 基因（glossy13）的一个强候选基因。发现在多个 EMS 诱导的等位基因中，GRMZM2G118243 中存在提前终止密码子，并且 gl13 的一个敲除等位基因，验证了基因 GRMZM2G118243 是 gl13 的假设。与此一致，GRMZM2G118243 是拟南芥 AtABCG32、大麦 HvABCG31 和水稻 OsABCG31 的同源物，它们编码 ABCG 亚家族转运蛋白，参与各种次生代谢物的跨膜运输。因此，我们假设 gl13 参与将角质层蜡质运输到幼苗叶片表面。
