Jafarian A, Sadeghi M R, Pejhan N, Salehkhou S, Lakpour N, Akhondi M M
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Andrologia. 2014 Dec;46(10):1098-106. doi: 10.1111/and.12198. Epub 2013 Dec 11.
Busulfan is a chemotherapeutic drug that induces sterility, azoospermia and testicular atrophy. To induce degeneration of spermatogenesis, we used different amounts of busulfan. Adult male C57Bl/6 mice were treated with 15, 30 and 45 mg kg(-1) of busulfan. After 5 weeks, animals had daily injections of 7.5 IU human follicle-stimulating hormone (hFSH) and 12.5 μg kg(-1) oestradiol benzoate (EB), separately or simultaneously. After this time, the animals were killed and blood samples were taken through cardiac puncture. Testes were used for histopathology experiments, DNA flow cytometry and RNA extraction for expression of c-kit and cyclin B1 genes. EB unlike FSH has induced stimulatory effects on spermatogenesis, increased the level of serum testosterone 2-fold and caused a 2-fold increase in the number of haploid cells. The result showed that hFSH with EB multiplied EB stimulatory effects on spermatogenesis up to four times. Expression of c-kit and cyclin B1 genes increased in EB and hFSH+EB groups. These findings suggest that EB regulates spermatogonial stem cells via hFSH. hFSH with EB had synergistic effect on regeneration of spermatogenesis.
白消安是一种可导致不育、无精子症和睾丸萎缩的化疗药物。为诱导生精细胞退化，我们使用了不同剂量的白消安。对成年雄性C57Bl/6小鼠分别给予15、30和45 mg kg(-1)的白消安。5周后，每天分别或同时给动物注射7.5 IU人卵泡刺激素（hFSH）和12.5 μg kg(-1)苯甲酸雌二醇（EB）。此后，处死动物并通过心脏穿刺采集血样。睾丸用于组织病理学实验、DNA流式细胞术以及提取RNA以检测c-kit和细胞周期蛋白B1基因的表达。与FSH不同，EB对生精有刺激作用，使血清睾酮水平增加2倍，并使单倍体细胞数量增加2倍。结果表明，hFSH与EB联合使用可使EB对生精的刺激作用增强至四倍。EB组和hFSH+EB组中c-kit和细胞周期蛋白B1基因的表达增加。这些发现表明，EB通过hFSH调节精原干细胞。hFSH与EB对生精再生具有协同作用。
