Zhang Chi, Yang Xi, Zhang Qu, Yang Baixia, Xu Liping, Qin Qin, Zhu Hongcheng, Liu Jia, Cai Jing, Tao Guangzhou, Ma Jianxin, Ge Xiaolin, Zhang Sheng, Cheng Hongyan, Sun Xinchen
Department of Radiotherapy, the First Affiliated Hospital of Nanjing Medical University , Nanjing, China.
Acta Otolaryngol. 2014 Feb;134(2):185-92. doi: 10.3109/00016489.2013.850176. Epub 2013 Dec 10.
Berberine confers radiosensitivity on nasopharyngeal carcinoma (NPC) and this is associated with the down-regulation of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression. Berberine could be a promising radiosensitizer for NPC radiotherapy.
NPC has a poor prognosis. Radiotherapy as first-line therapy significantly increases patient survival but radioresistance is a problem. This study aimed to investigate the radiosensitizing effects of berberine on NPC and explore the underlying mechanisms.
CNE-1 and CNE-2 cells were exposed to hypoxia and treated with berberine at different concentrations. The MTT assay, clonogenic assay, and flow cytometry were performed to analyze cell proliferation, colony formation, and apoptosis. The expression of HIF-1α and VEGF was assessed by Western blot and immunofluorescence analysis. Male nude mice inoculated subcutaneously with CNE-2 cells were used to examine the sensitizing effects of berberine in vivo.
Berberine efficiently radiosensitized NPC cells and xenografts in mice, and inhibited hypoxia/radiation-induced up-regulation of HIF-1α and VEGF expression.
黄连素赋予鼻咽癌（NPC）放射敏感性，这与缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）表达下调有关。黄连素可能是一种有前景的鼻咽癌放疗增敏剂。
鼻咽癌预后较差。放疗作为一线治疗可显著提高患者生存率，但放射抗性是一个问题。本研究旨在探讨黄连素对鼻咽癌的放射增敏作用并探究其潜在机制。
将CNE-1和CNE-2细胞置于缺氧环境中，并用不同浓度的黄连素处理。进行MTT法、克隆形成试验和流式细胞术以分析细胞增殖、集落形成和凋亡情况。通过蛋白质免疫印迹法和免疫荧光分析评估HIF-1α和VEGF的表达。使用皮下接种CNE-2细胞的雄性裸鼠来检测黄连素在体内的增敏作用。
黄连素有效使鼻咽癌细胞和小鼠异种移植瘤对放射敏感，并抑制缺氧/辐射诱导的HIF-1α和VEGF表达上调。
