西班牙马德里分析病理学人类免疫缺陷病毒阳性患者中的人乳头瘤病毒基因型。

Human papillomavirus genotypes in human immunodeficiency virus-positive patients with anal pathology in Madrid, Spain.

机构信息

Department of Histology and Anatomical Pathology, Rey Juan Carlos University, School of Medicine, Madrid, Spain.

出版信息

Diagn Pathol. 2013 Dec 10;8:204. doi: 10.1186/1746-1596-8-204.

DOI:10.1186/1746-1596-8-204

PMID:24325764

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3937168/

Abstract

BACKGROUND

We studied anal specimens to determine the distribution of human papillomavirus (HPV) genotypes and co-infection occurrence. This information will contribute to the knowledge of HPV genotype distributions and provide an estimate of the prevalence of different oncogenic HPV genotypes found in patients in Madrid (Spain).

METHODS

We studied a total of 82 anal biopsies from the Hospital General Universitario Gregorio Marañón of Madrid. These included 4 specimens with benign lesions, 52 specimens with low-grade anal squamous intraepithelial lesion, 24 specimens with high-grade anal squamous intraepithelial lesions and 2 specimens with invasive anal carcinoma. HPV genotyping was performed with PCR amplification and reverse dot blot hybridization.

RESULTS

We detected 33 different HPV genotypes, including 16 HPVs associated with a high risk of carcinogenesis, 3 HPVs associated with a highly likely risk of carcinogenesis and 14 HPVs associated with a low-risk of carcinogenesis. In two specimens, an uncharacterized HPV genotype was detected. The most frequent HPV genotypes found were HPV-16 (10.3%; 95% CI: 6.6%-15.1%), HPV-52 (8.5%; 95% CI: 5.2%-13%) and HPV-43/44 (7.6%; 95% CI: 4.5%-11.9%). HPV-18 was only detected in 0.9% (95% CI: 0.1%-3.2%) of the total viruses detected in all lesions. HPV co-infections were found in 83.9% of all types of lesions. The majority of cases (90.2%) were concomitantly infected with the human immunodeficiency virus (HIV).

CONCLUSION

The prevalence of high-risk carcinogenic genotypes in anal pathological samples was remarkable. Therefore, further studies that include a greater number of samples, particularly invasive carcinoma cases are needed to evaluate the potential influence of these HPV genotypes in the appearance of anal carcinomas. Also, the influence of other accompanying infections should be evaluated clarify the appearance of this type of carcinoma.

VIRTUAL SLIDES

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2075238024106058.

摘要

背景

我们研究了肛门标本，以确定人乳头瘤病毒（HPV）基因型的分布和合并感染的发生情况。这些信息将有助于了解 HPV 基因型的分布，并估计在马德里（西班牙）的患者中发现的不同致癌 HPV 基因型的流行率。

方法

我们研究了马德里格雷戈里奥·马拉尼昂综合大学医院的总共 82 例肛门活检标本。其中包括 4 例良性病变标本、52 例低级别肛门鳞状上皮内病变标本、24 例高级别肛门鳞状上皮内病变标本和 2 例浸润性肛门癌标本。HPV 基因分型采用 PCR 扩增和反向斑点印迹杂交法进行。

结果

我们检测到 33 种不同的 HPV 基因型，包括 16 种与致癌风险高度相关的 HPV、3 种与高度可能致癌风险相关的 HPV 和 14 种与低致癌风险相关的 HPV。在两个标本中，检测到一种未确定的 HPV 基因型。最常见的 HPV 基因型是 HPV-16（10.3%；95%CI：6.6%-15.1%）、HPV-52（8.5%；95%CI：5.2%-13%）和 HPV-43/44（7.6%；95%CI：4.5%-11.9%）。HPV-18 仅在所有病变中检测到的总病毒的 0.9%（95%CI：0.1%-3.2%）中发现。在所有类型的病变中，HPV 合并感染率为 83.9%。大多数病例（90.2%）同时感染了人类免疫缺陷病毒（HIV）。