YY1 抑制 C2C12 细胞中 Runx2 的转录活性。

YY1 represses the transcriptional activity of Runx2 in C2C12 cells.

机构信息

College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, South Korea.

出版信息

Mol Cell Endocrinol. 2014 Mar 5;383(1-2):103-10. doi: 10.1016/j.mce.2013.12.001. Epub 2013 Dec 8.

DOI:10.1016/j.mce.2013.12.001

PMID:24325869

Abstract

Runx2 is a major transcription factor that induces osteoblast differentiation by bone morphogenetic proteins (BMPs). Conversely, YY1 is a transcription factor that inhibits BMP2-induced cell differentiation. Until now, there has been no understanding of how osteoblast differentiation by Runx2 and YY1 is regulated. In this study we focused on the relationship between Runx2 and YY1. We confirmed that alkaline phosphatase staining is repressed by YY1. Runx2 interacted with YY1 through Runt and the C-terminus domain of Runx2. YY1 markedly repressed the Runx2-mediated enhancement of transcriptional activity on the osteocalcin and alkaline phosphatase promoters. Knockdown of YY1 enhanced BMP2- and Runx2-induced osteoblast differentiation. YY1 decreased Runx2 DNA binding affinity. The results indicate that YY1 represses osteoblast differentiation by an interaction with Runx2 and inhibits the transcriptional activity of Runx2.

摘要

Runx2 是一种主要的转录因子，通过骨形态发生蛋白 (BMPs) 诱导成骨细胞分化。相反，YY1 是一种转录因子，可抑制 BMP2 诱导的细胞分化。到目前为止，人们还不了解 Runx2 和 YY1 如何调节成骨细胞分化。在这项研究中，我们专注于 Runx2 和 YY1 之间的关系。我们证实碱性磷酸酶染色受 YY1 抑制。Runx2 通过 Runt 和 Runx2 的 C 末端结构域与 YY1 相互作用。YY1 显著抑制了 Runx2 对骨钙素和碱性磷酸酶启动子的转录活性的增强作用。YY1 的敲低增强了 BMP2 和 Runx2 诱导的成骨细胞分化。YY1 降低了 Runx2 的 DNA 结合亲和力。结果表明，YY1 通过与 Runx2 的相互作用抑制成骨细胞分化，并抑制 Runx2 的转录活性。