Negative effect of P72 polymorphism on p53 gene in IVF outcome in patients with repeated implantation failure and pregnancy loss.

PURPOSE

Investigate whether R72P on p53 gene polymorphism has a higher prevalence among women with a history of recurrent implantation failure (RIF) and pregnancy loss (RPL) and its influence in their IVF cycle outcome.

MATERIAL AND METHODS

p53 polymorphism R72P has been studied in 181 women. The control group included 83 oocyte donors. In the study group 98 women were included: 44 with RIF and 54 with RPL. From the study group, 76 patients underwent IVF-cycles (55 RPL and 21 RIF).

RESULTS

The frequency of PP genotypes on p53 among RIF was 11.4% compared with 18.5% for RPL and 6% in controls (p < 0.01). There were no significant differences with respect to patient characteristics. Significant differences were reported in pregnancy rate (69.4% for RR/RP and 33.3% for PP; p < 0.05), embryo implantation rate (33.3% for RR/RP and 7.3% for PP; p < 0.05) and ongoing pregnancy rate (53.1% for RR/RP and 14.3% for PP; p < 0.05) among RIF and RPL.

CONCLUSIONS

This investigation reveals that in RIF and RPL patients R72P on p53 gene is more prevalent than fertile population. Moreover, patients carrying a PP genotype on p53 codon 72 will have less chance to achieve an ongoing pregnancy. This information together with some additional markers will allow development of diagnostic tests for detects risk for RIF and RPL before infertility treatment is initiated.