Flaherty S P, Winfrey V P, Olson G E
Anat Rec. 1986 Dec;216(4):504-15. doi: 10.1002/ar.1092160407.
The distribution of monomeric and polymeric actin in spermatozoa from the bull, boar, rabbit, human, rat, mouse, golden hamster, and guinea pig has been examined by using a monoclonal antiactin antibody and NBD-phallacidin. Actin was present in sperm from each species. When the monoclonal antibody was used, there was a species-specific distribution and intensity of fluorescence, but no generalized pattern. Specific fluorescence was noted in the neck and principal piece of human sperm; in the postacrosomal region, neck, and midpiece of bull and boar sperm; in the postacrosomal region, neck, and principal/equatorial segment border of rabbit sperm; in the neck region of hamster sperm; and in the neck, midpiece, and principal piece of rat, mouse, and guinea pig sperm. Sperm from all eight species displayed no specific fluorescence with NBD-phallacidin, indicating that actin was present in a nonfilamentous form. SDS extracts of sperm were analyzed by SDS-PAGE and Western blotting; in sperm from each species, a 42-kD protein with specific affinity for the monoclonal antibody was present.
利用单克隆抗肌动蛋白抗体和NBD-鬼笔环肽，对公牛、公猪、兔子、人类、大鼠、小鼠、金黄仓鼠和豚鼠精子中单体和聚合肌动蛋白的分布进行了检测。每个物种的精子中都存在肌动蛋白。使用单克隆抗体时，荧光的分布和强度存在物种特异性，但没有普遍模式。在人类精子的颈部和主段观察到特异性荧光；在公牛和公猪精子的顶体后区域、颈部和中段；在兔子精子的顶体后区域、颈部和主段/赤道段边界；在仓鼠精子的颈部区域；以及在大鼠、小鼠和豚鼠精子的颈部、中段和主段。所有八个物种的精子用NBD-鬼笔环肽处理后均未显示特异性荧光，表明肌动蛋白以非丝状形式存在。通过SDS-PAGE和蛋白质免疫印迹法分析精子的SDS提取物；每个物种的精子中都存在一种对单克隆抗体具有特异性亲和力的42-kD蛋白。
