Ara h 6作为花生IgE反应性的重要标志物，对Ara h 2起到补充作用。
Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut.
作者信息
Koid Audrey E, Chapman Martin D, Hamilton Robert G, van Ree Ronald, Versteeg Serge A, Dreskin Stephen C, Koppelman Stef J, Wünschmann Sabina
机构信息
INDOOR Biotechnologies, Incorporated , 1216 Harris Street, Charlottesville, Virginia 22903, United States.
出版信息
J Agric Food Chem. 2014 Jan 8;62(1):206-13. doi: 10.1021/jf4022509. Epub 2013 Dec 26.
Abstract
The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2. The objectives of this study were to purify natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the highly purified allergen (<0.01% Ara h 2) revealed a single 14.5 kD band, and the identity of Ara h 6 was confirmed by liquid chromatography-tandem mass spectrometry. Ara h 6 showed a higher seroprevalence in chimeric IgE enzyme-linked immunosorbent assay (n = 54) but a weaker biological activity in basophil histamine release assays than Ara h 2. Purified Ara h 6 will be useful for diagnostic IgE antibody assays as well as molecular and cellular studies to investigate the immunological mechanisms of peanut allergy.
摘要
两种主要花生过敏原Ara h 2和Ara h 6在分子大小、氨基酸序列和结构上的相似性使得难以获得不含Ara h 2的天然Ara h 6。本研究的目的是纯化基本不含Ara h 2的天然Ara h 6，并在组胺释放试验中比较其与Ara h 2的IgE反应性和效力。高度纯化的过敏原（<0.01% Ara h 2）的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示出一条单一的14.5 kD条带，通过液相色谱-串联质谱法确认了Ara h 6的身份。在嵌合IgE酶联免疫吸附试验（n = 54）中，Ara h 6显示出更高的血清阳性率，但在嗜碱性粒细胞组胺释放试验中其生物活性比Ara h 2弱。纯化的Ara h 6将有助于诊断性IgE抗体检测以及研究花生过敏免疫机制的分子和细胞研究。
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