Department of Biochemistry and Molecular Pharmacology, IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , Via La Masa 19, 20156 Milan, Italy.
ACS Nano. 2014 Jan 28;8(1):175-87. doi: 10.1021/nn402669w. Epub 2013 Dec 16.
This study describes the formulation optimization and body-cell distribution and clearance in mice of a dually fluorescent biodegradable poly avidin nanoassembly based on the novel Avidin-Nucleic-Acid-Nano-ASsembly (ANANAS) platform as a potential advancement of classic avidin/biotin-based targeted delivery. The nanoformulation circulates freely in the bloodstream; it is slowly captured by filter organs; it is efficiently cleared within 24-48 h, and it is poorly immunogenic. The system displays more favorable properties than its parent monomeric avidin and it is a promising tool for diagnostic purposes for future translational aims, for which free circulation in the bloodstream, safety, multifunctionality and high composition definition are all necessary requirements. In addition, the assembly shows a time-dependent cell penetration capability, suggesting it may also function as a NP-dependent drug delivery tool. The ease of preparation together with the possibility to fine-tune the surface composition makes it also an ideal candidate to understand if and how nanoparticle composition affects its localization.
本研究描述了一种新型基于 Avidin-Nucleic-Acid-Nano-ASsembly（ANANAS）平台的双重荧光可生物降解聚亲和素纳米组装体的配方优化及其在小鼠体内的细胞分布和清除情况，作为经典亲和素/生物素靶向递药的潜在进展。该纳米制剂在血液中自由循环；它被滤过器官缓慢捕获；它在 24-48 小时内被有效清除，且免疫原性差。该系统比其母体单体亲和素有更优越的性能，有望成为未来转化目标的诊断工具，其在血液中自由循环、安全性、多功能性和高成分定义都是必要的要求。此外，该组装体显示出时间依赖性的细胞穿透能力，表明它也可能作为一种 NP 依赖性药物递送工具。其易于制备以及能够精细调整表面成分的特点，使其也成为一个理想的候选者，可以了解纳米颗粒的组成是否以及如何影响其定位。
