Plessers L, Bosmans E, Cox A, Raus J
Anticancer Res. 1986 Sep-Oct;6(5):885-8.
Spleen cells from Balb/c mice immunized with human breast cancer cells (MCF-7) were fused with murine myeloma SP2/0 cells. Screening of the monoclonal antibodies produced was carried out on glutaraldehyde fixed cells coated on microtiterplates. An initial evaluation of the specificity was obtained by comparing the binding of the monoclonal antibodies to MCF-7 cells with the binding to human peripheral blood lymphocytes. Eight monoclonal antibodies reacting with different epitopes on the MCF-7 cells were obtained. On the basis of their clonal origin, isotype and reaction pattern towards the MCF-7 cells these monoclonal antibodies were subdivided into two classes. Both groups of antibodies reacted with fixed and unfixed MCF-7 cells. The cellular distribution of the antigens recognized by the monoclonal antibodies was determined. To check for specificity a panel of different cells (of human and animal origin) was evaluated by immunocytochemical techniques.
用人类乳腺癌细胞（MCF - 7）免疫的Balb/c小鼠的脾细胞与鼠骨髓瘤SP2/0细胞融合。对产生的单克隆抗体进行筛选是在包被于微量滴定板上的戊二醛固定细胞上进行的。通过比较单克隆抗体与MCF - 7细胞的结合以及与人类外周血淋巴细胞的结合，对其特异性进行了初步评估。获得了8种与MCF - 7细胞上不同表位反应的单克隆抗体。根据它们的克隆起源、同种型以及对MCF - 7细胞的反应模式，这些单克隆抗体被分为两类。两组抗体均与固定和未固定的MCF - 7细胞反应。确定了单克隆抗体所识别抗原的细胞分布。为检查特异性，通过免疫细胞化学技术对一组不同的细胞（人类和动物来源）进行了评估。
