Sandhya B, Seetharamappa J
a Department of Chemistry , Karnatak University , Dharwad , Karnataka , India.
Nucleosides Nucleotides Nucleic Acids. 2013;32(12):660-9. doi: 10.1080/15257770.2013.851392.
The interaction of an anti-HIV drug, stavudine (STV) with calf thymus deoxyribonucleic acid (DNA) was investigated employing acridine orange (AO) as a fluorescence probe. Spectroscopic investigations revealed the intercalative mode of binding of STV to DNA. The analysis of fluorescence data indicated the presence of static quenching mechanism between STV and DNA. Thermodynamic parameters indicated the presence of van der Waals forces in addition to intercalative mode of binding. CD data revealed the partial B → A conformational transition of DNA upon intercalative mode of binding with STV.
使用吖啶橙（AO）作为荧光探针，研究了抗艾滋病毒药物司他夫定（STV）与小牛胸腺脱氧核糖核酸（DNA）的相互作用。光谱研究揭示了STV与DNA的嵌入结合模式。荧光数据分析表明STV与DNA之间存在静态猝灭机制。热力学参数表明除了嵌入结合模式外还存在范德华力。圆二色性（CD）数据显示，在与STV嵌入结合模式下，DNA发生了部分B→A构象转变。
