Safdari Yaghoub, Farajnia Safar, Asgharzadeh Mohammad, Khosroshahi Shiva Ahdi, Veisi Kamal, Ahmadzadeh Vahideh, Khalili Masoumeh
1 Biotechnology Research Center, Tabriz University of Medical Sciences , Tabriz, Iran .
Monoclon Antib Immunodiagn Immunother. 2013 Dec;32(6):409-12. doi: 10.1089/mab.2013.0049.
The current study was aimed at introducing an efficient enzyme-conjugated molecule for use in the detection of humanized single chain antibodies. Because they are composite in their variable domain sequences and also devoid of constant domains, humanized single chain antibodies require a suitable secondary enzyme-conjugated antibody (more precisely enzyme-conjugated protein molecule) to be efficiently recognized and detected in ELISA, dot blot, and other detection tests, guaranteeing a more precise evaluation of their quantity, affinity, and other features. In the current study we examined the ability of three HRP-conjugated protein molecules including protein L, anti-human IgG antibody, and anti-mouse IgG antibody in the detection of three humanized single chain antibodies (anti-CD20, anti-EGFRvIII, and anti-EGFR) in ELISA and dot blot tests. The results signify the HRP-conjugated protein L as the most efficient molecule for detecting humanized single chain antibody.
本研究旨在引入一种高效的酶偶联分子，用于检测人源化单链抗体。由于人源化单链抗体的可变域序列复杂且缺乏恒定域，因此在ELISA、斑点印迹和其他检测试验中，需要合适的二级酶偶联抗体（更准确地说是酶偶联蛋白分子）才能有效地识别和检测它们，从而保证对其数量、亲和力和其他特性进行更精确的评估。在本研究中，我们检测了三种HRP偶联蛋白分子，即蛋白L、抗人IgG抗体和抗小鼠IgG抗体，在ELISA和斑点印迹试验中检测三种人源化单链抗体（抗CD20、抗EGFRvIII和抗EGFR）的能力。结果表明，HRP偶联蛋白L是检测人源化单链抗体最有效的分子。
