Cui Jing, Liu Yuanyuan, Zhang Jingkai, Yan Hua
1 Department of Ophthalmology, Tianjin Medical University General Hospital , Tianjin, China .
Photomed Laser Surg. 2014 Jan;32(1):30-6. doi: 10.1089/pho.2013.3588. Epub 2013 Dec 14.
The purpose of this work was to study the efficacy and safety of choroidal neovascularization (CNV) formation induced by Krypton laser in Brown Norway (BN) rats, and observe the trend of the change of CNV after laser photocoagulation.
Twenty-five male BN rats were involved in this study. Two eyes of one rat without any laser photocoagulation were randomly selected as the control group, and the other 48 eyes of 24 rats were selected as the experimental group. Eight eyes of four rats were randomly selected to receive the examinations of fundus fluorescein angiography (FFA), histopathology, and transmission electron microscopy 3, 7, 14, 21, 28, and 56 days after laser photocoagulation.
After laser photocoagulation, the leakage appeared in burns on day 7 (59%), reached the peak on day 21 (84%), (p<0.01), and remained stable after day 21, proven by FFA and histopathology (p>0.05). The thickness of CNV increased from day 7 to day 21 (p<0.01), and remained stable after day 21 (p>0.05).
The experimental model of CNV can be successfully induced by Krypton laser in rats with a stable, long-lasting, and high success rate. After laser photocoagulation, the leakages appear on day 7, reach the peak on day 21, and remain stable after day 21.
本研究旨在探讨氪激光诱导棕色挪威（BN）大鼠脉络膜新生血管（CNV）形成的有效性和安全性，并观察激光光凝后CNV的变化趋势。
25只雄性BN大鼠参与本研究。随机选取1只大鼠未经任何激光光凝的2只眼作为对照组，其余24只大鼠的48只眼作为实验组。随机选取4只大鼠的8只眼在激光光凝后3、7、14、21、28和56天进行眼底荧光血管造影（FFA）、组织病理学和透射电子显微镜检查。
激光光凝后，第7天（59%）烧伤处出现渗漏，第21天达到高峰（84%），（p<0.01），21天后保持稳定，FFA和组织病理学证实（p>0.05）。CNV厚度从第7天到第21天增加（p<0.01），21天后保持稳定（p>0.05）。
氪激光可在大鼠中成功诱导出稳定、持久且成功率高的CNV实验模型。激光光凝后，渗漏在第7天出现，第21天达到高峰，21天后保持稳定。
