Chiarugi V P, Pasquali F, Vannucchi S, Ruggiero M
Biochem Biophys Res Commun. 1986 Dec 15;141(2):591-9. doi: 10.1016/s0006-291x(86)80214-5.
A high-affinity muscarinic receptor is detectable both in normal 3T3 mouse fibroblasts and in their transformed counterpart obtained by transfection with the oncogene EJ/T24-H-ras. However, only the transformed cell line is responsive to muscarinic agonist carbamylcholine in terms of Ca2+ influx and polyphosphoinositide hydrolysis, whereas the normal cell line is unresponsive. Using a point-mutated p21ras protein and monoclonal antibodies anti-p21ras, we provide evidences that p21ras couples to receptor-operating calcium channels and to polyphosphoinositide hydrolysis a muscarinic receptor which is uncoupled in normal mouse fibroblasts.
在正常的3T3小鼠成纤维细胞及其通过癌基因EJ/T24-H-ras转染获得的转化细胞中均能检测到高亲和力的毒蕈碱受体。然而，就Ca2+内流和多磷酸肌醇水解而言，只有转化细胞系对毒蕈碱激动剂氨甲酰胆碱有反应，而正常细胞系无反应。利用点突变的p21ras蛋白和抗p21ras单克隆抗体，我们提供证据表明p21ras与受体操纵的钙通道以及与正常小鼠成纤维细胞中未偶联的毒蕈碱受体的多磷酸肌醇水解偶联。
