Hajizadeh Moghaddam Akbar, Bigdellu Rata, Fatemi Tabatabaei Seyed Reza, Roohbakhsh Ali
Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
Department of Basic Science, Faculty of Veterinary of Medicine, Shahid Chamran, Ahvaz, Iran.
Arch Iran Med. 2013 Dec;16(12):711-6.
A large body of evidence suggests that the cannabinoid CB1 receptor plays a key role in the regulation of emotional behaviors. The present study was designed to evaluate the effects of CB1 agonist and antagonist on anxiety-like behaviors in the lateral septum (LS) region of the rat brain using elevated plus maze test.
Rats were anesthetized with ketamine and xylazine and special cannulas were inserted stereotaxically into the LS region. After 1 week of recovery, the effects of intra-LS administration of the CB1 receptor agonist, WIN 55,212-2 and CB1 receptor antagonist, AM251, on %OAT and %OAE were measured. Moreover, the effects of pretreatment with AM251 on the response induced by intra-LS administration of WIN 55,212-2 were also assessed.
Intra-LS administration of WIN 55,212-2 (0.001, 0.005 and 0.5μg/rat) decreased the %OAT and %OAE but not locomotor activity, showing an anxiogenic-like response. Intra-LS injection of different doses of AM251 (0.001, 0.01 and 0.1 µg/rat) did not significantly alter the anxiety-like parameters on the plus-maze test. However, intra-LS injections of AM251 (0.01 µg/rat) significantly reversed WIN 55,212-2-induced anxiogenic-like effects.
The results suggest that the cannabinoid system of the lateral septum modulates anxiety-like behavior through CB1 receptor.
大量证据表明，大麻素CB1受体在情绪行为调节中起关键作用。本研究旨在通过高架十字迷宫试验评估CB1激动剂和拮抗剂对大鼠脑外侧隔（LS）区域焦虑样行为的影响。
用氯胺酮和赛拉嗪麻醉大鼠，并通过立体定位将特殊套管插入LS区域。恢复1周后，测量向LS区域内注射CB1受体激动剂WIN 55,212-2和CB1受体拮抗剂AM251对开放臂时间百分比（%OAT）和开放臂进入次数百分比（%OAE）的影响。此外，还评估了用AM251预处理对向LS区域内注射WIN 55,212-2所诱导反应的影响。
向LS区域内注射WIN 55,212-2（0.001、0.005和0.5μg/只大鼠）可降低%OAT和%OAE，但不影响运动活性，表现出焦虑样反应。向LS区域内注射不同剂量的AM251（0.001、0.01和0.1μg/只大鼠）对高架十字迷宫试验中的焦虑样参数无显著影响。然而，向LS区域内注射AM251（0.01μg/只大鼠）可显著逆转WIN 55,212-2诱导的焦虑样效应。
结果表明，外侧隔的大麻素系统通过CB1受体调节焦虑样行为。
