Tian Na, Liu Shuoqian, Li Juan, Xu Wenwen, Yuan Lin, Huang Jianan, Liu Zhonghua
Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Hardening, Hunan Agricultural University, Changsha, China.
Physiol Plant. 2014 Aug;151(4):522-32. doi: 10.1111/ppl.12139. Epub 2014 Jan 27.
Adventitious root (AR) formation is a critical process for plant clonal propagation. The role of plant secondary metabolites in AR formation is still poorly understood. Chemical and physical mutagenesis in combination with somatic variation were performed on Artemisia annua in order to obtain a mutant with changes in adventitious rooting and composition of plant secondary metabolites. Metabolic and morphological analyses of the iar (increased adventitious rooting) mutant coupled with in vitro assays were used to elucidate the relationship between plant secondary metabolites and AR formation. The only detected differences between the iar mutant and wild-type were rooting capacity and borneol/camphor content. Consistent with this, treatment with borneol in vitro promoted adventitious rooting in wild-type. The enhanced rooting did not continue upon removal of borneol. The iar mutant displayed no significant differences in AR formation upon treatment with camphor. Together, our results suggest that borneol promotes adventitious rooting whereas camphor has no effect on AR formation.
不定根（AR）形成是植物克隆繁殖的关键过程。植物次生代谢产物在不定根形成中的作用仍知之甚少。为了获得不定根和植物次生代谢产物组成发生变化的突变体，对黄花蒿进行了化学和物理诱变并结合体细胞变异。通过对iar（不定根增加）突变体进行代谢和形态分析并结合体外试验，以阐明植物次生代谢产物与不定根形成之间的关系。iar突变体与野生型之间唯一检测到的差异是生根能力和冰片/樟脑含量。与此一致的是，体外使用冰片处理可促进野生型不定根的形成。去除冰片后，增强的生根作用不再持续。用樟脑处理后，iar突变体在不定根形成方面没有显著差异。总之，我们的结果表明，冰片促进不定根形成，而樟脑对不定根形成没有影响。
