The identification of inhibitors in bromelin one-stage irregular antibody screening techniques.
作者信息
Ogasawara K, Mazda T, Shimizu M
出版信息
Blut. 1987 Jan;54(1):51-5. doi: 10.1007/BF00326028.
PMID:2432972
Abstract
Human sera contain bromelin inhibitors in irregular antibody screening techniques. In order to identify and purify these inhibitors, pooled human sera were precipitated with ammonium sulfate, followed by DEAE-cellulose and Sephadex G-150 column chromatography. Proteinase activity and inhibitory activity to bromelin were observed in two protein fractions, these were identified as alpha 2-macroglobulin and the S-2 thiol proteinase inhibitor.
摘要
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