Bai Hanako, Sakurai Toshihiro, Godkin James D, Imakawa Kazuhiko
Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Anim Sci J. 2014 Apr;85(4):388-94. doi: 10.1111/asj.12156. Epub 2013 Dec 12.
In vertebrates, six GATA transcription factors, GATA1 through GATA6, have been identified and GATA1-3 is known to be involved in hematopoietic developments, while GATA4-6 play roles in cardiac and endoderm developments. Recently, we and others have found that GATA2 and GATA3 found in the trophectoderm plays a role in gene expression specific to this cell type, but GATA4-6 have not been well characterized in early embryonic developments. Using quantitative polymerase chain reaction (qPCR) and in situ hybridization, we examined the expression of GATA4, 5 and 6 messenger RNAs (mRNAs) in ovine conceptuses and uteri during the peri-implantation period. In ovine conceptuses, GATA4, 5 and 6 transcripts were present on days 15, 17 and 21 (day 0 = day of mating), and high GATA5 and 6 mRNAs were found on day 21, most of which were localized in the trophectoderm and endoderm. Moreover, minute and substantial GATA4 and 5 mRNAs were found in days 15 and 21 uterine endometria, respectively. Increase in GATA4-6 transcripts in day 21 uteri indicates that in addition to GATA1-3, GATA4-6 may also play a potentially novel role in the development of ovine trophectoderm, endoderm and/or uterine endometria following conceptus attachment to the uterine epithelium.
在脊椎动物中，已鉴定出六种GATA转录因子，即GATA1至GATA6，已知GATA1 - 3参与造血发育，而GATA4 - 6在心脏和内胚层发育中发挥作用。最近，我们和其他人发现，滋养外胚层中的GATA2和GATA3在这种细胞类型特有的基因表达中起作用，但GATA4 - 6在早期胚胎发育中的特征尚未明确。我们使用定量聚合酶链反应（qPCR）和原位杂交技术，检测了着床前后绵羊胚胎和子宫中GATA4、5和6信使核糖核酸（mRNA）的表达。在绵羊胚胎中，GATA4、5和6转录本在第15、17和21天出现（第0天 = 交配日），在第21天发现高表达的GATA5和6 mRNA，其中大部分定位于滋养外胚层和内胚层。此外，分别在第15天和第21天的子宫子宫内膜中发现了微量和大量的GATA4和5 mRNA。第21天子宫中GATA4 - 6转录本的增加表明，除了GATA1 - 3之外，GATA4 - 6在胚胎附着于子宫上皮后绵羊滋养外胚层、内胚层和/或子宫子宫内膜的发育中可能也发挥着潜在的新作用。
