Colin S, Kruger L
Brain Res. 1986 Nov 19;398(1):199-203. doi: 10.1016/0006-8993(86)91270-9.
A whole-mount preparation is described for visualizing unmyelinated peptide immunoreactive axons in two specialized tissues, thin enough for tracing axons to their terminal knobs. These tissues, the cornea and the mucosal surface of the tympanic membrane, are known to possess specialized nociceptor innervation and can be used for selecting sensory endings labeled by peroxidase reaction product and embedded in plastic for more detailed analysis. Rat calcitonin gene-related peptide antibody proved a particularly robust primary antibody found in a substantial proportion of sensory unmyelinated axons in a variety of fixation and preparative conditions.
本文描述了一种整装标本制备方法，用于观察两种特殊组织中无髓鞘的肽免疫反应性轴突，这两种组织足够薄，能够追踪轴突直至其终末小结。已知角膜和鼓膜黏膜表面这两种组织具有特殊的伤害性感受器神经支配，可用于挑选出由过氧化物酶反应产物标记并包埋于塑料中以便进行更详细分析的感觉末梢。在多种固定和制备条件下，大鼠降钙素基因相关肽抗体被证明是一种特别有效的一抗，在相当比例的感觉无髓鞘轴突中均可发现。
