Dellagi K, Lipinski M, Paulin D, Portier M M, Lenoir G M, Brouet J C
Cancer Res. 1987 Feb 15;47(4):1170-3.
The histogenesis of Ewing sarcoma is still controversial; we therefore studied the expression of intermediate filaments (IF) in cell lines derived from Ewing tumors since identification of IF in tumor cells is considered a reliable marker of tissue origin and differentiation. All nine lines studied expressed vimentin IF; in addition, a small number of Ewing cells from three lines expressed keratin filaments. After treatment with phorbol esters, a high percentage of cells from these three lines synthesize keratin IF identified by immunoblotting as keratin 8 and 18 polypeptides, which are expressed by single epithelia and epithelial cells in early embryonic development. Furthermore cells from a fourth line synthesize keratins after transplantation in nude mice. These data indicate that, under certain conditions, undifferentiated Ewing cells may acquire an IF phenotype related to that of epithelial cells.
尤因肉瘤的组织发生仍存在争议；因此，我们研究了源自尤因肿瘤的细胞系中中间丝（IF）的表达情况，因为肿瘤细胞中IF的鉴定被认为是组织起源和分化的可靠标志物。所研究的九条细胞系均表达波形蛋白中间丝；此外，来自三条细胞系的少数尤因细胞表达角蛋白丝。在用佛波酯处理后，这三条细胞系中的高比例细胞合成了经免疫印迹鉴定为角蛋白8和18多肽的角蛋白中间丝，这些角蛋白由早期胚胎发育中的单层上皮和上皮细胞表达。此外，第四条细胞系的细胞在裸鼠体内移植后合成角蛋白。这些数据表明，在某些条件下，未分化的尤因细胞可能获得与上皮细胞相关的中间丝表型。
