Lefort-Tran M, Bre M H, Pouphile M, Manigault P
Cytometry. 1987 Jan;8(1):46-54. doi: 10.1002/cyto.990080108.
Vitamin B12 starvation in Euglena induces a cell cycle arrest that leads to unbalanced growth. Microfluorometry and flow cytometry analyses of cellular DNA fluorescence after Hoechst 33258 staining were performed on control and vitamin B12-deficient cells. Convergent results are obtained with both methods. Histograms that represent arrested cells are unimodal, with a mode channel value nearly twice that of the G1 control cell peak. Dispersion of fluorescence values is great, and values from 2C and over 4C are observed and discussed. It appears that vitamin B12 starvation in Euglena leads to defective DNA synthesis. Blocked cells have different DNA content, corresponding to blockade of DNA replication during the S phase. A second block prevents the onset of mitosis even for 4C cells.
眼虫属中的维生素B12缺乏会导致细胞周期停滞，进而导致生长失衡。对对照细胞和缺乏维生素B12的细胞进行了Hoechst 33258染色后细胞DNA荧光的微量荧光测定和流式细胞术分析。两种方法得到了一致的结果。代表停滞细胞的直方图是单峰的，其峰值通道值几乎是G1对照细胞峰值的两倍。荧光值的离散度很大，观察并讨论了2C及超过4C的值。似乎眼虫属中的维生素B12缺乏会导致DNA合成缺陷。受阻细胞具有不同的DNA含量，这与S期DNA复制的阻断相对应。第二个阻断甚至会阻止4C细胞进入有丝分裂。
