Macario A J, Dugan C B, Conway de Macario E
J Bacteriol. 1987 Feb;169(2):666-9. doi: 10.1128/jb.169.2.666-669.1987.
Eight well-characterized Methanogenium strains, including the six described type strains, were analyzed with poly- and monoclonal antibody probes to examine the antigenic mosaic of the genus. The pattern of cross-reactions showed that the mosaic is complex and varies with the strains; thus, these organisms have developed a considerable antigenic diversity, which is expressed in their envelopes. Every strain shared at least one determinant with at least one other strain, demonstrating the antigenic cohesiveness of the group. This finding, together with the fact that most strains displayed a distinctive antigenic fingerprint (notwithstanding the limited number of probes available), emphasizes the potential of antibodies for rapid identification of new isolates and for direct elucidation of Methanogenium strains in microbial mixtures.
对8株特征明确的产甲烷菌菌株（包括6株已描述的模式菌株）进行了分析，使用多克隆和单克隆抗体探针来研究该属的抗原镶嵌性。交叉反应模式表明，这种镶嵌性很复杂且因菌株而异；因此，这些生物体已形成了相当大的抗原多样性，这在它们的包膜中有所体现。每株菌株至少与另一株菌株共享一个决定簇，表明该类群具有抗原凝聚性。这一发现，再加上大多数菌株显示出独特的抗原指纹（尽管可用的探针数量有限），强调了抗体在快速鉴定新分离株以及直接阐明微生物混合物中产甲烷菌菌株方面的潜力。
